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Quantification of extracted rna samples based on one-step rt-qpcr. the

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Rnase if -qpcr assay overview. the total rna is isolated first (aMolecular validation of nuclear rna enrichment by rt-qpcr and rna-seq Addgene: kit free rna extractionMouse adipose tissue collection and processing for rna analysis.

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(a) qpcr was performed with total rna extracted from conditional strain

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